A quantitative comparison of cell-type-specific microarray gene expression profiling methods in the mouse brain.

ABSTRACT: Expression profiling of restricted neural populations using microarrays can facilitate neuronal classification and provide insight into the molecular bases of cellular phenotypes. Due to the formidable heterogeneity of intermixed cell types that make up the brain, isolating cell types prior to microarray processing poses steep technical challenges that have been met in various ways. These methodological differences have the potential to distort cell-type-specific gene expression profiles insofar as they may insufficiently filter out contaminating mRNAs or induce aberrant cellular responses not normally present in vivo. Thus we have compared the repeatability, susceptibility to contamination from off-target cell-types, and evidence for stress-responsive gene expression of five different pur

SUBMITTER: Okaty BW 

PROVIDER: S-EPMC3029380 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature