Cortical actin dynamics facilitate early-stage centrosome separation.

ABSTRACT: Proper centrosome separation is a prerequisite for positioning the bipolar spindle. Although studies demonstrate that microtubules (MTs) and their associated motors drive centrosome separation [1], the role of actin in centrosome separation remains less clear. Studies in tissue culture cells indicate that actin- and myosin-based cortical flow is primarily responsible for driving late centrosome separation [2], whereas other studies suggest that actin plays a more passive role by serving as an attachment site for astral MTs to pull centrosomes apart [3-6]. Here we demonstrate that prior to nuclear envelope breakdown (NEB) in Drosophila embryos, proper centrosome separation does not require myosin II but requires dynamic actin rearrangements at the growing edge of the interphase cap. Both Ar

SUBMITTER: Cao J 

PROVIDER: S-EPMC3032811 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature