Absence of Vsx1 expression in the normal and damaged mouse cornea.

ABSTRACT:

Purpose

To examine the expression of visual system homeobox 1 (Vsx1) in the mouse cornea and its potential role in the corneal wound response pathway.

Methods

Expression of Vsx1 was examined by quantitative reverse-transcription PCR (qRT-PCR) in corneal tissue from developing and adult mice and from mice that had undergone alkali-burn corneal wounding. Immunolabeling and Vsx1 knock-in reporter gene expression in wild type and Vsx1 null-mice were used to confirm the qRT-PCR data.

Results

Using qRT-PCR, Vsx1 expression was not detected in either the postnatal or adult mouse cornea or in corneas following wounding. This qRT-PCR data was supported by the absence of specific Vsx1 immunolabeling and Vsx1 knock-in reporter expression in untreated and wounded corneas.

Conc

SUBMITTER: Watson T 

PROVIDER: S-EPMC3062518 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature