Lactate dehydrogenase A promotes communication between carbohydrate catabolism and virulence in Bacillus cereus.

ABSTRACT: The diarrheal potential of a Bacillus cereus strain is essentially dictated by the amount of secreted nonhemolytic enterotoxin (Nhe). Expression of genes encoding Nhe is regulated by several factors, including the metabolic state of the cells. To identify metabolic sensors that could promote communication between central metabolism and nhe expression, we compared four strains of the B. cereus group in terms of metabolic and nhe expression capacities. We performed growth performance measurements, metabolite analysis, and mRNA measurements of strains F4430/73, F4810/72, F837/76, and PA cultured under anoxic and fully oxic conditions. The results showed that expression levels of nhe and ldhA, which encodes lactate dehydrogenase A (LdhA), were correlated in both aerobically and anaerobically g

SUBMITTER: Laouami S 

PROVIDER: S-EPMC3067659 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature