Novel serum proteomic signatures in a non-human primate model of retinal injury.

ABSTRACT:

Purpose

To identify candidate protein biomarkers in sera indicative of acute retinal injury.

Methods

We used laser photocoagulation as a model of acute retinal injury in Rhesus macaques. In a paired-control study design, we collected serum from each animal (n=6) at 4 h, 1 day, and 3 days following a mock procedure and then again following retinal laser treatment that produced mild lesions. Samples were fractionated by isoelectric focusing, digested with trypsin, and analyzed by liquid chromatography/tandem mass spectrometry (LC-MS/MS). Spectral counting was used to determine relative protein abundances and identify proteins with statistically significant differences between control and treated sera.

Results

Mild retinal injury was confirmed by fundus photography and h

SUBMITTER: Dunmire JJ 

PROVIDER: S-EPMC3081796 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature