Investigation of LGI1 as the antigen in limbic encephalitis previously attributed to potassium channels: a case series.

ABSTRACT:

Background

Voltage-gated potassium channels are thought to be the target of antibodies associated with limbic encephalitis. However, antibody testing using cells expressing voltage-gated potassium channels is negative; hence, we aimed to identify the real autoantigen associated with limbic encephalitis.

Methods

We analysed sera and CSF of 57 patients with limbic encephalitis and antibodies attributed to voltage-gated potassium channels and 148 control individuals who had other disorders with or without antibodies against voltage-gated potassium channels. Immunohistochemistry, immunoprecipitation, and mass spectrometry were used to characterise the antigen. An assay with HEK293 cells transfected with leucine-rich, glioma-inactivated 1 (LGI1) and disintegrin and metalloprotein

SUBMITTER: Lai M 

PROVIDER: S-EPMC3086669 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature