A mouse model of cholestasis-associated cholangiocarcinoma and transcription factors involved in progression.

ABSTRACT:

Background & aims

Cholestasis contributes to hepatocellular injury and promotes liver carcinogenesis. We created a mouse model of chronic cholestasis to study its effects on progression of cholangiocarcinoma and the oncogenes involved.

Methods

To induce chronic cholestasis, Balb/c mice were given 2 weekly intraperitoneal injections of diethylnitrosamine (DEN); 2 weeks later, some mice also received left and median bile duct ligation (LMBDL) and, then 1 week later, were fed DEN, in corn oil, weekly by oral gavage (DLD). Liver samples were analyzed by immunohistochemical and biochemical assays; expression of Mnt and c-Myc was reduced by injection of small inhibitor RNAs.

Results

Chronic cholestasis was induced by DLD and accelerated progression of cholangiocarcinoma, co

SUBMITTER: Yang H 

PROVIDER: S-EPMC3129489 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature