A screen for peptide agonists of the G-CSF receptor.

ABSTRACT:

Background

Granulocyte-colony stimulating factor (G-CSF) is one of the most important pharmacologically used proteins. Potential uses beyond the stimulation of neutrophilic granulocytes are the treatment of CNS disorders. Disadvantages of the G-CSF protein as a drug are its moderate plasma half-life time and considerable production costs. We therefore conducted a screen for peptide agonists derived from the sequence of human G-CSF.

Findings

Despite of the high sensitivity of our screening system we could not detect any positive hits in a single peptide approach. In a multiplex approach using a permutation of any combination of 10 different peptides we could also not detect a positive block.

Conclusions

We conclude that larger coherent parts of the protein or dimerisin

SUBMITTER: Conzelmann N 

PROVIDER: S-EPMC3132715 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature