Salvage and storage of infectious disease protein targets in the SSGCID high-throughput crystallization pathway using microfluidics.

ABSTRACT: The MPCS Plug Maker is a microcapillary-based protein-crystallization system for generating diffraction-ready crystals from nanovolumes of protein. Crystallization screening using the Plug Maker was used as a salvage pathway for proteins that failed to crystallize during the initial observation period using the traditional sitting-drop vapor-diffusion method. Furthermore, the CrystalCards used to store the crystallization experiments set up by the Plug Maker are shown be a viable container for long-term storage of protein crystals without a discernable loss of diffraction quality with time. Use of the Plug Maker with SSGCID proteins is demonstrated to be an effective crystal-salvage and storage method.

SUBMITTER: Christensen J 

PROVIDER: S-EPMC3169396 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature