Aldosterone decreases glucose-stimulated insulin secretion in vivo in mice and in murine islets.

ABSTRACT:

Aims/hypothesis

Aldosterone concentrations increase in obesity and predict the onset of diabetes. We investigated the effects of aldosterone on glucose homeostasis and insulin secretion in vivo and in vitro.

Methods

We assessed insulin sensitivity and insulin secretion in aldosterone synthase-deficient (As [also known as Cyp11b2](-/-)) and wild-type mice using euglycaemic-hyperinsulinaemic and hyperglycaemic clamps, respectively. We also conducted studies during high sodium intake to normalise renin activity and potassium concentration in As (-/-) mice. We subsequently assessed the effect of aldosterone on insulin secretion in vitro in the presence or absence of mineralocorticoid receptor antagonists in isolated C57BL/6J islets and in the MIN6 beta cell line.

Results

SUBMITTER: Luther JM 

PROVIDER: S-EPMC3216479 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature