Comparative analysis of nuclear transfer embryo-derived mouse embryonic stem cells. Part II: gene regulation.

ABSTRACT: In a mouse model nuclear transfer embryo-derived embryonic stem cell lines (ntESCs) of various genetic backgrounds and donor cell types were compared with reference ESCs and analyzed comprehensively at molecular level as a second part of a larger study. Expression profiles of ntESCs established by different NT-methods (piezoelectric microinjection or zona-free) were indistinguishable. However, expression profiling analyses identified differentially regulated genes between reference ESCs and ntESCs from different genetic backgrounds. A number of pluripotency and stemness marker genes significantly differed at the mRNA level between the cell lines. However, cluster and lineage analyses revealed that such differences had no effect on cell differentiation and cell fate. Regardless of the donor

SUBMITTER: Kobolak J 

PROVIDER: S-EPMC3275096 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature