"Point" centromeres of Saccharomyces harbor single centromere-specific nucleosomes.

ABSTRACT: The "point" centromere of budding yeast is genetically defined by an ≈ 125-bp sequence. Recent fluorescence measurements of kinetochore clusters have suggested that this sequence specifies multiple centromere histone 3 (CenH3) nucleosomes. However, high-resolution mapping demonstrates that there is only one CenH3 nucleosome per centromere, providing biochemical confirmation of the point centromere model.

SUBMITTER: Henikoff S 

PROVIDER: S-EPMC3316665 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature