Thyroid hormone enhances the ability of serum to accept cellular cholesterol via the ABCA1 transporter.

ABSTRACT:

Objective

The goal of this study was to examine the effects of thyroid hormone status on the ability of serum to accept cellular cholesterol.

Methods and results

Sera from hypophysectomized rats treated ± T(3) was used to evaluate the role of thyroid hormone on serum efflux capacity. 2D-DIGE analysis of serum proteins showed that T(3) treated rats had increased ApoA-I, ApoA-IV and fetuin A levels with decreased Apo E levels. Microarray and real-time RT-PCR analysis of rat liver revealed large increases in ApoA-I, ApoA-IV, ABCG5, and ABCG8 in response to T(3). J774 macrophages, BHK cells, and Fu5AH rat hepatoma cells were used to measure cholesterol efflux mediated by ABCA1, ABCG1 transporters or SR-BI. Sera from T(3)-treated rats stimulated efflux via ABCA1 but not by ABCG1

SUBMITTER: Boone LR 

PROVIDER: S-EPMC3324859 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature