Identification of RALDH2 as a visually regulated retinoic acid synthesizing enzyme in the chick choroid.

ABSTRACT:

Purpose

All-trans-retinoic acid (atRA) has been implicated in the local regulation of scleral proteoglycan synthesis in vivo. The purpose of the present study was to identify the enzymes involved in the synthesis of atRA during visually guided ocular growth, the cells involved in modulation of atRA biosynthesis in the choroid, and the effect of choroid-derived atRA on scleral proteoglycan synthesis.

Methods

Myopia was induced in White leghorn chicks by form deprivation for 10 days, followed by up to 15 days of unrestricted vision (recovery). Expression of atRA synthesizing enzymes was evaluated by semiquantitative qRT-PCR, in situ hybridization, and immunohistochemistry. atRA synthesis was measured in organ cultures of isolated choroids using LC-tandem MS quantification. Scl

SUBMITTER: Rada JA 

PROVIDER: S-EPMC3339922 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature