A critical role for muscle ring finger-1 in acute lung injury-associated skeletal muscle wasting.

ABSTRACT:

Rationale

Acute lung injury (ALI) is a debilitating condition associated with severe skeletal muscle weakness that persists in humans long after lung injury has resolved. The molecular mechanisms underlying this condition are unknown.

Objectives

To identify the muscle-specific molecular mechanisms responsible for muscle wasting in a mouse model of ALI.

Methods

Changes in skeletal muscle weight, fiber size, in vivo contractile performance, and expression of mRNAs and proteins encoding muscle atrophy-associated genes for muscle ring finger-1 (MuRF1) and atrogin1 were measured. Genetic inactivation of MuRF1 or electroporation-mediated transduction of miRNA-based short hairpin RNAs targeting either MuRF1 or atrogin1 were used to identify their role in ALI-associated skele

SUBMITTER: Files DC 

PROVIDER: S-EPMC3360571 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature