Bioconjugates of PAMAM dendrimers with trans-retinal, pyridoxal, and pyridoxal phosphate.

ABSTRACT:

Background

Bioconjugates of a polyamidoamine (PAMAM) G3 dendrimer and an aldehyde were synthesized as carriers for vitamins A and B₆, and the bioavailability of these vitamins for skin nutrition was investigated.

Methods

Nuclear magnetic resonance (NMR) and ultraviolet-visible methods were used to characterize the structure of the bioconjugates and for monitoring release of pyridoxal (Pyr) and pyridoxal phosphate (PLP) from these bioconjugates in vitro. A skin model permeation of bioconjugates was also studied in a Franz chamber.

Results

A transdermal G3 PAMAM dendrimer was used to synthesize bioconjugates with trans-retinal (Ret), pyridoxal (Pyr), or PLP. These nanomolecules, containing up to four covalently linked Ret, Pyr, or PLP (G3(4Ret), G3(4Pyr), and G3(4PLP)),

SUBMITTER: Filipowicz A 

PROVIDER: S-EPMC3439857 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature