Sanger sequencing in routine KRAS testing: a review of 1720 cases from a pathologist's perspective.

ABSTRACT:

Background

Sanger sequencing (SS) of PCR products is still the most frequent method to test colorectal cancer for KRAS mutations in routine practice.

Methods

An audit of SS on 1720 routine cases was carried out, taking into account age, gender, specimen type (resection vs biopsies), tumour site (primary vs metastasis), tumour stage, neoplastic cells abundance (>30% vs <30%) and fixation type (buffered formalin vs simple formalin). In a subset of 50 wild-type (WT) patients correlations between SS findings and response rate (RR), progression-free survival (PFS) and overall survival (OS) were also evaluated.

Results

The tests were informative in 1691 cases (98.3%). Mutations were detected in 671 cases (39.6%). No significant differences in mutation rates were observed wi

SUBMITTER: Malapelle U 

PROVIDER: S-EPMC3461636 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature