Hepatitis B viral core protein disrupts human host gene expression by binding to promoter regions.

ABSTRACT:

Background

The core protein (HBc) of hepatitis B virus (HBV) has been implicated in the malignant transformation of chronically-infected hepatocytes and displays pleiotropic functions, including RNA- and DNA-binding activities. However, the mechanism by which HBc interacts with the human genome to exert effects on hepatocyte function remains unknown. This study investigated the distribution of HBc binding to promoters in the human genome and evaluated its effects on the related genes' expression.

Results

Whole-genome chromatin immunoprecipitation microarray (ChIP-on-chip) analysis was used to identify HBc-bound human gene promoters. Gene Ontology and pathway analyses were performed on related genes. The quantitative polymerase chain reaction assay was used to verify ChIP-on-

SUBMITTER: Guo Y 

PROVIDER: S-EPMC3484065 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature