Strategy for the creation of clinical grade hESC line banks that HLA-match a target population.
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ABSTRACT:
SUBMITTER: Jacquet L
PROVIDER: S-EPMC3569650 | biostudies-literature | 2013 Jan
REPOSITORIES: biostudies-literature
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Strategy for the creation of clinical grade hESC line banks that HLA-match a target population.
Jacquet Laureen L Stephenson Emma E Collins Robert R Patel Heema H Trussler Jane J Al-Bedaery Roaa R Renwick Pamela P Ogilvie Caroline C Vaughan Robert R Ilic Dusko D
EMBO molecular medicine 20121119 1
Here, we describe a pre-derivation embryo haplotyping strategy that we developed in order to maximize the efficiency and minimize the costs of establishing banks of clinical grade hESC lines in which human leukocyte antigen (HLA) haplotypes match a significant proportion of the population. Using whole genome amplification followed by medium resolution HLA typing using PCR amplification with sequence-specific primers (PCR-SSP), we have typed the parents, embryos and hESC lines from three families ...[more]
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