Coordinated induction of multi-gene pathways in Saccharomyces cerevisiae.

ABSTRACT: Bacterial operons are nature's tool for regulating and coordinating multi-gene expression in prokaryotes. They are also a gene architecture commonly used in the biosynthesis of many pharmaceutically important compounds and industrially useful chemicals. Despite being an important eukaryotic production host, Saccharomyces cerevisiae has never had such gene architecture. Here, we report the development of a system to assemble and regulate a multi-gene pathway in S. cerevisiae. Full pathways can be constructed using pre-made parts from a plasmid toolbox. Subsequently, through the use of a yeast strain containing a stably integrated gene switch, the assembled pathway can be regulated using a readily available and inexpensive compound-estradiol-with extremely high sensitivity (10 nM). To demons

SUBMITTER: Liang J 

PROVIDER: S-EPMC3581276 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature