Directly transforming PCR-amplified DNA fragments into plant cells is a versatile system that facilitates the transient expression assay.

ABSTRACT: A circular plasmid containing a gene coding sequence has been broadly used for studying gene regulation in cells. However, to accommodate a quick screen plasmid construction and preparation can be time consuming. Here we report a PCR amplified dsDNA fragments (PCR-fragments) based transient expression system (PCR-TES) for suiting in the study of gene regulation in plant cells. Instead of transforming plasmids into plant cells, transient expression of PCR-fragments can be applicable. The transformation efficiency and expression property of PCR-fragments are comparable to transformation using plasmids. We analyzed the transformation efficiency in PCR-TES at transcription and protein levels. Our results indicate that the PCR-TES is as versatile as the conventional transformation system using

SUBMITTER: Lu Y 

PROVIDER: S-EPMC3582559 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature