Endocrine-Disrupting Chemicals (EDCs): In Vitro Mechanism of Estrogenic Activation and Differential Effects on ER Target Genes.

ABSTRACT:

Background

Endocrine-disrupting chemicals (EDCs) influence the activity of estrogen receptors (ERs) and alter the function of the endocrine system. However, the diversity of EDC effects and mechanisms of action are poorly understood.

Objectives

We examined the agonistic activity of EDCs through ERα and ERβ. We also investigated the effects of EDCs on ER-mediated target genes.

Methods

HepG2 and HeLa cells were used to determine the agonistic activity of EDCs on ERα and ERβ via the luciferase reporter assay. Ishikawa cells stably expressing ERα were used to determine changes in endogenous ER target gene expression by EDCs.

Results

Twelve EDCs were categorized into three groups on the basis of product class and similarity of chemical structure. As shown by lucifer

SUBMITTER: Li Y 

PROVIDER: S-EPMC3620735 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature