Experimental interrogation of the path dependence and stochasticity of protein evolution using phage-assisted continuous evolution.

ABSTRACT: To what extent are evolutionary outcomes determined by a population's recent environment, and to what extent do they depend on historical contingency and random chance? Here we apply a unique experimental system to investigate evolutionary reproducibility and path dependence at the protein level. We combined phage-assisted continuous evolution with high-throughput sequencing to analyze evolving protein populations as they adapted to divergent and then convergent selection pressures over hundreds of generations. Independent populations of T7 RNA polymerase genes were subjected to one of two selection histories ("pathways") demanding recognition of distinct intermediate promoters followed by a common final promoter. We observed distinct classes of solutions with unequal phenotypic activity a

SUBMITTER: Dickinson BC 

PROVIDER: S-EPMC3670371 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature