Transcriptome based identification of mouse cumulus cell markers that predict the developmental competence of their enclosed antral oocytes.

ABSTRACT:

Background

The cumulus cells (CCs) enveloping antral and ovulated oocytes have been regarded as putative source of non-invasive markers of the oocyte developmental competence. A number of studies have indeed observed a correlation between CCs gene expression, embryo quality, and final pregnancy outcome. Here, we isolated CCs from antral mouse oocytes of known developmental incompetence (NSN-CCs) or competence (SN-CCs) and compared their transcriptomes with the aim of identifying distinct marker transcripts.

Results

Global gene expression analysis highlighted that both types of CCs share similar transcriptomes, with the exception of 422 genes, 97.6% of which were down-regulated in NSN-CCs vs. SN-CCs. This transcriptional down-regulation in NSN-CCs was confirmed by qRT-PCR ana

SUBMITTER: Vigone G 

PROVIDER: S-EPMC3679864 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature