Characterization of differential transcript abundance through time during Nematostella vectensis development.

ABSTRACT:

Background

Nematostella vectensis, a burrowing sea anemone, has become a popular species for the study of cnidarian development. In previous studies, the expression of a variety of genes has been characterized during N. vectensis development with in situ mRNA hybridization. This has provided detailed spatial resolution and a qualitative perspective on changes in expression. However, little is known about broad transcriptome-level patterns of gene expression through time. Here we examine the expression of N. vectensis genes through the course of development with quantitative RNA-seq. We provide an overview of changes in the transcriptome through development, and examine the maternal to zygotic transition, which has been difficult to investigate with other tools.

Results

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SUBMITTER: Helm RR 

PROVIDER: S-EPMC3680055 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature