Analysis and prediction of pathways in HeLa cells by integrating biological levels of organization with systems-biology approaches.

ABSTRACT: It has recently begun to be considered that cancer is a systemic disease and that it must be studied at every level of complexity using many of the currently available approaches, including high-throughput technologies and bioinformatics. To achieve such understanding in cervical cancer, we collected information on gene, protein and phosphoprotein expression of the HeLa cell line and performed a comprehensive analysis of the different signaling pathways, transcription networks and metabolic events in which they participate. A total expression analysis by RNA-Seq of the HeLa cell line showed that 19,974 genes were transcribed. Of these, 3,360 were over-expressed, and 2,129 under-expressed when compared to the NHEK cell line. A protein-protein interaction network was derived from the over-ex

SUBMITTER: Higareda-Almaraz JC 

PROVIDER: S-EPMC3680226 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature