Transcriptional and epigenetic signatures of zygotic genome activation during early Drosophila embryogenesis.

ABSTRACT:

Background

In all Metazoa, transcription is inactive during the first mitotic cycles after fertilisation. In Drosophila melanogaster, Zygotic Genome Activation (ZGA) occurs in two waves, starting respectively at mitotic cycles 8 (approximately 60 genes) and 14 (over a thousand genes). The regulatory mechanisms underlying these drastic transcriptional changes remain largely unknown.

Results

We developed an original gene clustering method based on discretized transition profiles, and applied it to datasets from three landmark early embryonic transcriptome studies. We identified 417 genes significantly up-regulated during ZGA. De novo motif discovery returned nine motifs over-represented in their non-coding sequences (upstream, introns, UTR), three of which correspond to previo

SUBMITTER: Darbo E 

PROVIDER: S-EPMC3706223 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature