Follicular lymphoma cells induce changes in T-cell gene expression and function: potential impact on survival and risk of transformation.

ABSTRACT:

Purpose

Previous studies have demonstrated the prognostic importance of the immune microenvironment in follicular lymphoma (FL). To investigate the molecular mechanisms during which tumor-infiltrating T cells (TILs) are altered in the FL microenvironment, we studied highly purified CD4 and CD8 TILs from lymph node biopsies at diagnosis in treatment-naive patients with FL compared with reactive tonsils and the peripheral blood of healthy donors.

Patients and methods

Gene expression profiling of highly purified CD4 and CD8 TILs was performed on the Affymetrix platform. Diagnostic tissue microarrays from an independent patient set (n = 172) were used to verify protein expression and analyze any impact of TIL-expressed genes on outcome. Time-lapse imaging was used to assess T-ce

SUBMITTER: Kiaii S 

PROVIDER: S-EPMC3709054 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature