Ontology highlight
ABSTRACT:
SUBMITTER: Larochelle S
PROVIDER: S-EPMC3746743 | biostudies-literature | 2012 Nov
REPOSITORIES: biostudies-literature
Nature structural & molecular biology 20121014 11
Promoter-proximal pausing by RNA polymerase II (Pol II) ensures gene-specific regulation and RNA quality control. Structural considerations suggested a requirement for initiation-factor eviction in elongation-factor engagement and pausing of transcription complexes. Here we show that selective inhibition of Cdk7--part of TFIIH--increases TFIIE retention, prevents DRB sensitivity-inducing factor (DSIF) recruitment and attenuates pausing in human cells. Pause release depends on Cdk9-cyclin T1 (P-T ...[more]