Potential limitations of the NSG humanized mouse as a model system to optimize engineered human T cell therapy for cancer.

ABSTRACT: The genetic modification of peripheral blood lymphocytes using retroviral vectors to redirect T cells against tumor cells has been recently used as a means to generate large numbers of antigen-specific T cells for adoptive cell therapy protocols. However, commonly used retroviral vector-based genetic modification requires T cells to be driven into cell division; this potent mitogenic stimulus is associated with the development of an effector phenotype that may adversely impact upon the long-term engraftment potential and subsequent antitumor effects of T cells. To investigate whether the cytokines used during culture impact upon the engraftment potential of gene-modified T cells, a humanized model employing T cells engrafted with a MART-1-specific T cell receptor adoptively transferred int

SUBMITTER: Alcantar-Orozco EM 

PROVIDER: S-EPMC3798231 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature