Tear cytokine profile as a noninvasive biomarker of inflammation for ocular surface diseases: standard operating procedures.

ABSTRACT:

Purpose

To provide standard operating procedures (SOPs) for measuring tear inflammatory cytokine concentrations and to validate the resulting profile as a minimally invasive objective metric and biomarker of ocular surface inflammation for use in multicenter clinical trials on dry eye disease (DED).

Methods

Standard operating procedures were established and then validated with cytokine standards, quality controls, and masked tear samples collected from local and distant clinical sites. The concentrations of the inflammatory cytokines in tears were quantified using a high-sensitivity human cytokine multiplex kit.

Results

A panel of inflammatory cytokines was initially investigated, from which four key inflammatory cytokines (IL-1β, IL-6, INF-γ, and TNF-α) were chosen.

SUBMITTER: Wei Y 

PROVIDER: S-EPMC3875391 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature