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Sleep drives metabolite clearance from the adult brain.

ABSTRACT: The conservation of sleep across all animal species suggests that sleep serves a vital function. We here report that sleep has a critical function in ensuring metabolic homeostasis. Using real-time assessments of tetramethylammonium diffusion and two-photon imaging in live mice, we show that natural sleep or anesthesia are associated with a 60% increase in the interstitial space, resulting in a striking increase in convective exchange of cerebrospinal fluid with interstitial fluid. In turn, convective fluxes of interstitial fluid increased the rate of ?-amyloid clearance during sleep. Thus, the restorative function of sleep may be a consequence of the enhanced removal of potentially neurotoxic waste products that accumulate in the awake central nervous system.

SUBMITTER: Xie L

PROVIDER: S-EPMC3880190 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature

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Sleep drives metabolite clearance from the adult brain.

Xie Lulu L Kang Hongyi H Xu Qiwu Q Chen Michael J MJ Liao Yonghong Y Thiyagarajan Meenakshisundaram M O'Donnell John J Christensen Daniel J DJ Nicholson Charles C Iliff Jeffrey J JJ Takano Takahiro T Deane Rashid R Nedergaard Maiken M

Science (New York, N.Y.) 20131001 6156

The conservation of sleep across all animal species suggests that sleep serves a vital function. We here report that sleep has a critical function in ensuring metabolic homeostasis. Using real-time assessments of tetramethylammonium diffusion and two-photon imaging in live mice, we show that natural sleep or anesthesia are associated with a 60% increase in the interstitial space, resulting in a striking increase in convective exchange of cerebrospinal fluid with interstitial fluid. In turn, conv ...[more]

PMID: 24136970

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