Hypoxia-induced deregulation of miR-126 and its regulative effect on VEGF and MMP-9 expression.

ABSTRACT:

Objective

miR-126, the miRNA considered to be specially expressed in endothelial cells and hematopoietic progenitor cells, is strongly associated with angiogenesis. The purpose is to evaluate the role of miR-126 in hypoxia-induced angiogenesis and the possible mechanisms.

Methods

The expression of miR-126 was detected in hypoxia-treated RF/6A cells and diabetic retinas using real-time PCR. The miR-126 was up- or down-regulated by transfecting miR-126-mimics or inhibitors into RF/6A cells. Cell cycle analysis was performed using flow cytometry. The protein levels of vascular endothelial growth factor (VEGF) and matrix metalloproteinase-9 (MMP-9) were assessed by immunoblotting.

Results

A significantly decreased expression of miR-126 was found in hypoxia-treated RF/6A c

SUBMITTER: Ye P 

PROVIDER: S-EPMC3880987 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature