An eEF1A1 truncation encoded by PTI-1 exerts its oncogenic effect inside the nucleus.

ABSTRACT:

Background

The oncogene PTI-1 was originally isolated from a prostate cancer cell line by its capability to transform rat fibroblasts. The PTI-1 mRNA has a very eccentric structure as the 5'UTR is similar to prokaryotic 23S rRNA, while the major open reading frame and the 3'UTR corresponds to a part of the mRNA encoding human translation elongation factor eEF1A1. Thus, the largest open reading frame encodes a truncated version of eEF1A1 lacking the first 67 amino acids, while having three unique N-terminal amino acids. Previously, the UTRs were shown to be a prerequisite for the transforming capacity of the PTI-1 transcript. In this study, we have investigated the possible role of the UTRs in regulating protein expression and localization.

Methods

The protein expression prof

SUBMITTER: Dahl LD 

PROVIDER: S-EPMC3941776 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature