One-step generation of knockout pigs by zygote injection of CRISPR/Cas system.
Ontology highlight
ABSTRACT:
SUBMITTER: Hai T
PROVIDER: S-EPMC3945887 | biostudies-literature | 2014 Mar
REPOSITORIES: biostudies-literature
Publications
One-step generation of knockout pigs by zygote injection of CRISPR/Cas system.
Hai Tang T Teng Fei F Guo Runfa R Li Wei W Zhou Qi Q
Cell research 20140131 3
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Project description:Triple-knockout mice generated by the one-step CRISPR/Cas9 system were examined for the effects of multiple gene modifications on each phenotype and individual gene function. Sixty embryos were transferred, and 9 pups were obtained; all 9 pups had mutations on 3 loci, and 7 pups showed mutations in all-alleles. F0 mice showed knockout phenotypes or no protein expression of target genes simultaneously, and these mutations were normally inherited in the next generation.
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