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A simple cost-effective methodology for large-scale purification of recombinant non-animal collagens.


ABSTRACT: Recently, a different class of collagen-like molecules has been identified in numerous bacteria. Initial studies have shown that these collagens are readily produced in Escherichia coli and they have been isolated and purified by various small-scale chromatography approaches. These collagens are non-cytotoxic, are non-immunogenic, and can be produced in much higher yields than mammalian collagens, making them potential new collagens for biomedical materials. One of the major drawbacks with large-scale fermentation of collagens has been appropriate scalable down-stream processing technologies. Like other collagens, the triple helical domains of bacterial collagens are particularly resistant to proteolysis. The present study describes the development and optimization of a simple, scalable procedure using a combination of acid precipitation of the E. coli host proteins, followed by proteolysis of residual host proteins to produce purified collagens in large scale without the use of chromatographic methods.

SUBMITTER: Peng YY 

PROVIDER: S-EPMC3968793 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature

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A simple cost-effective methodology for large-scale purification of recombinant non-animal collagens.

Peng Yong Y YY   Stoichevska Violet V   Madsen Soren S   Howell Linda L   Dumsday Geoff J GJ   Werkmeister Jerome A JA   Ramshaw John A M JA  

Applied microbiology and biotechnology 20140109 4


Recently, a different class of collagen-like molecules has been identified in numerous bacteria. Initial studies have shown that these collagens are readily produced in Escherichia coli and they have been isolated and purified by various small-scale chromatography approaches. These collagens are non-cytotoxic, are non-immunogenic, and can be produced in much higher yields than mammalian collagens, making them potential new collagens for biomedical materials. One of the major drawbacks with large  ...[more]

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