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Phenotype and regulation of immunosuppressive Vδ2-expressing γδ T cells.

ABSTRACT: The proliferation and interleukin-2 production of CD4(+)CD25(-) αβ T cells were inhibited in a cell-contact manner by Vδ2 γδ T cells. The transcription factor Helios was constitutively expressed in about one-third of circulating γδ T cells and was upregulated by CD28-signaling. Our data suggest that Helios could serve as a marker for differential activation status rather than for regulatory T cells (Treg). Our findings also indicate that the interaction of CD86 on activated Vδ2 T cells and cytotoxic T lymphocyte-associated antigen-4 (CTLA-4) on activated αβ T cells mediated the suppression because the suppressive effect was abolished by blocking the CD86:CTLA-4 interaction. Pre-treatment of Vδ2 T cells with Toll-like receptor 2 ligands enhanced phosphorylation of MAPKs, Akt, and NF-κB and partially abrogated the suppressive capacity, whereas on co-cultured responder T cells inhibitory molecules were downregulated and Akt and NF-κB phosphorylation was restored. Our results suggest that the regulation of αβ T cell proliferation by activated Vδ2 T cells might contribute to fine-tuning of αβ T cell responses.

SUBMITTER: Peters C

PROVIDER: S-EPMC3997799 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature

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Phenotype and regulation of immunosuppressive Vδ2-expressing γδ T cells.

Peters Christian C Oberg Hans-Heinrich HH Kabelitz Dieter D Wesch Daniela D

Cellular and molecular life sciences : CMLS 20131004 10

The proliferation and interleukin-2 production of CD4(+)CD25(-) αβ T cells were inhibited in a cell-contact manner by Vδ2 γδ T cells. The transcription factor Helios was constitutively expressed in about one-third of circulating γδ T cells and was upregulated by CD28-signaling. Our data suggest that Helios could serve as a marker for differential activation status rather than for regulatory T cells (Treg). Our findings also indicate that the interaction of CD86 on activated Vδ2 T cells and cytot ...[more]

PMID: 24091816

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Dataset Information

Phenotype and regulation of immunosuppressive Vδ2-expressing γδ T cells.

Publications

Phenotype and regulation of immunosuppressive Vδ2-expressing γδ T cells.

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