Yeast surface display for protein engineering and characterization.

ABSTRACT: Yeast surface display is being employed to engineer desirable properties into proteins for a broad variety of applications. Labeling with soluble ligands enables rapid and quantitative analysis of yeast-displayed libraries by flow cytometry, while cell-surface selections allow screening of libraries with insoluble or even as-yet-uncharacterized binding targets. In parallel, the utilization of yeast surface display for protein characterization, including in particular the mapping of functional epitopes mediating protein-protein interactions, represents a significant recent advance.

SUBMITTER: Gai SA 

PROVIDER: S-EPMC4038029 | biostudies-literature |

REPOSITORIES: biostudies-literature