Regulation of ACVR1 and ID2 by cell-secreted exosomes during follicle maturation in the mare.

ABSTRACT:

Background

Ovarian follicle growth and maturation requires extensive communication between follicular somatic cells and oocytes. Recently, intercellular cell communication was described involving cell-secreted vesicles called exosomes (50-150 nm), which contain miRNAs and protein, and have been identified in ovarian follicular fluid. The goal of this study was to identify a possible role of exosomes in follicle maturation.

Methods

Follicle contents were collected from mares at mid-estrous (~35 mm, before induction of follicular maturation) and pre-ovulatory follicles (30-34 h after induction of follicular maturation). A real time PCR screen was conducted to reveal significant differences in the presence of exosomal miRNAs isolated from mid-estrous and pre-ovulatory follicles

SUBMITTER: da Silveira JC 

PROVIDER: S-EPMC4045866 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature