Changes in granulosa cells gene expression associated with growth, plateau and atretic phases in medium bovine follicles.

ABSTRACT: BACKGROUND: The objective of this study was to build the transcriptomic profile of granulosa cells originating from follicles 6 to 9 mm in diameter in dairy cattle using microarrays. METHODS: Granulosa cells originating from three different phases of antral follicle growth were compared: growing (G), plateau (P) and atresia (A), as categorized by flow cytometry profiles of DNA. The growing and atretic conditions were each hybridized against the plateau condition as a reference in order to understand the specific biological mechanisms modulated in this class of follicles. RESULTS: 2,942 genes were differentially expressed (P?

SUBMITTER: Douville G 

PROVIDER: S-EPMC4046060 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature