Changes in granulosa cells gene expression associated with growth, plateau and atretic phases in medium bovine follicles.

ABSTRACT:

Background

The objective of this study was to build the transcriptomic profile of granulosa cells originating from follicles 6 to 9 mm in diameter in dairy cattle using microarrays.

Methods

Granulosa cells originating from three different phases of antral follicle growth were compared: growing (G), plateau (P) and atresia (A), as categorized by flow cytometry profiles of DNA. The growing and atretic conditions were each hybridized against the plateau condition as a reference in order to understand the specific biological mechanisms modulated in this class of follicles.

Results

2,942 genes were differentially expressed (P < 0.05) in P vs. G and 1,974 in A vs. P. A clear segregation of the 3 phases was confirmed by between group analysis (BGA). The first characteristic

SUBMITTER: Douville G 

PROVIDER: S-EPMC4046060 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature