A 3D sphere culture system containing functional polymers for large-scale human pluripotent stem cell production.

ABSTRACT: Utilizing human pluripotent stem cells (hPSCs) in cell-based therapy and drug discovery requires large-scale cell production. However, scaling up conventional adherent cultures presents challenges of maintaining a uniform high quality at low cost. In this regard, suspension cultures are a viable alternative, because they are scalable and do not require adhesion surfaces. 3D culture systems such as bioreactors can be exploited for large-scale production. However, the limitations of current suspension culture methods include spontaneous fusion between cell aggregates and suboptimal passaging methods by dissociation and reaggregation. 3D culture systems that dynamically stir carrier beads or cell aggregates should be refined to reduce shearing forces that damage hPSCs. Here, we report a simpl

SUBMITTER: Otsuji TG 

PROVIDER: S-EPMC4050473 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature