Lysyl oxidase-mediated collagen crosslinks may be assessed as markers of functional properties of tendon tissue formation.

ABSTRACT: Mechanical property elaboration of engineered tissues is often assumed on the basis of gene and protein characterizations, rather than mechanical testing. However, we recently demonstrated that mechanical properties are not consistently correlated with matrix content and organization during embryonic tissue development. Based on this, mechanical properties should be assessed independently during natural or engineered tissue formation. Unfortunately, mechanical testing is destructive, and thus alternative means of assessing these properties are desirable. In this study, we examined lysyl oxidase (LOX)-mediated crosslinks as markers for mechanical properties during embryonic tendon formation and the potential to detect them non-destructively. We used tandem mass spectrometry (LC-MS/MS) to qu

SUBMITTER: Marturano JE 

PROVIDER: S-EPMC4053294 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature