Split luciferase complementation assay to detect regulated protein-protein interactions in rice protoplasts in a large-scale format.

ABSTRACT:

Background

The rice interactome, in which a network of protein-protein interactions has been elucidated in rice, is a useful resource to identify functional modules of rice signal transduction pathways. Protein-protein interactions occur in cells in two ways, constitutive and regulative. While a yeast-based high-throughput method has been widely used to identify the constitutive interactions, a method to detect the regulated interactions is rarely developed for a large-scale analysis.

Results

A split luciferase complementation assay was applied to detect the regulated interactions in rice. A transformation method of rice protoplasts in a 96-well plate was first established for a large-scale analysis. In addition, an antibody that specifically recognizes a carboxyl-terminal f

SUBMITTER: Fujikawa Y 

PROVIDER: S-EPMC4077619 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature