Analysis of differentially expressed proteins in cancerous and normal colonic tissues.

ABSTRACT:

Aim

To identify and analyze the differentially expressed proteins in normal and cancerous tissues of four patients suffering from colon cancer.

Methods

Colon tissues (normal and cancerous) were homogenized and the proteins were extracted using three protein extraction buffers. The extraction buffers were used in an orderly sequence of increasing extraction strength for proteins with hydrophobic properties. The protein extracts were separated using the SDS-PAGE method and the images were captured and analyzed using Quantity One software. The target protein bands were subjected to in-gel digestion with trypsin and finally analyzed using an ESI-ion trap mass spectrometer.

Results

A total of 50 differentially expressed proteins in colonic cancerous and normal tissues were

SUBMITTER: Gam LH 

PROVIDER: S-EPMC4087399 | biostudies-literature | 2006 Aug

REPOSITORIES: biostudies-literature