Dataset Information

Rectal nitric oxide as biomarker in the treatment of inflammatory bowel disease: responders versus nonresponders.

ABSTRACT:

Aim

To explore rectal nitric oxide (NO) as biomarker of treatment response in ulcerative colitis (UC) and Crohn's disease (CD), and examine relationships between rectal NO, mucosal expression of NO synthases (NOS), and pro-inflammatory cytokines.

Methods

Twenty-two patients with UC and 24 with CD were monitored during steroid treatment. Rectal NO levels were measured and clinical activities were assessed on days 1, 3, 7 and 28. Mucosal presence of NOS and pro-inflammatory cytokines were analyzed by immunohistochemistry and RT-PCR.

Results

Active UC and CD displayed markedly increased rectal NO levels (10950 +/- 7610 and 5040 +/- 1280 parts per billion (ppb), respectively) as compared with the controls (154 +/- 71 ppb, P < 0.001). Rectal NO correlated weakly with disease activity in both UC and CD (r = 0.34 for UC and r = 0.48 for CD, P < 0.01). In 12 patients, a steroid-refractory course led to colectomy. These patients had only slightly increased NO levels (UC: 620 +/- 270 ppb; CD: 1260 +/- 550 ppb) compared to those with a therapeutic response (UC: 18860 +/- 530 ppb, P < 0.001; CD: 10060 +/- 3200 ppb, P < 0.05).

Conclusion

Rectal NO level is a useful biomarker of treatment response in IBD as low NO levels predicts a poor clinical response to steroid treatment.

SUBMITTER: Ljung T

PROVIDER: S-EPMC4087870 | biostudies-literature | 2006 Jun

REPOSITORIES: biostudies-literature

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Publications

Rectal nitric oxide as biomarker in the treatment of inflammatory bowel disease: responders versus nonresponders.

Ljung Tryggve T Lundberg Sofie S Varsanyi Mark M Johansson Catharina C Schmidt Peter T PT Herulf Max M Lundberg Jon O JO Hellstrom Per M PM

World journal of gastroenterology 20060601 21

<h4>Aim</h4>To explore rectal nitric oxide (NO) as biomarker of treatment response in ulcerative colitis (UC) and Crohn's disease (CD), and examine relationships between rectal NO, mucosal expression of NO synthases (NOS), and pro-inflammatory cytokines.<h4>Methods</h4>Twenty-two patients with UC and 24 with CD were monitored during steroid treatment. Rectal NO levels were measured and clinical activities were assessed on days 1, 3, 7 and 28. Mucosal presence of NOS and pro-inflammatory cytokine ...[more]

PMID: 16733856

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Dataset Information

Rectal nitric oxide as biomarker in the treatment of inflammatory bowel disease: responders versus nonresponders.

Aim

Methods

Results

Conclusion

Publications

Rectal nitric oxide as biomarker in the treatment of inflammatory bowel disease: responders versus nonresponders.

Similar Datasets

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