A comprehensive investigation of RHD polymorphisms in the Chinese Han population in Xi'an.

ABSTRACT:

Background

This study is a comprehensive analysis of RHD in D-negative phenotypes in saline, in Xi'an, Shanxi province, central China.

Material and methods

DCcEe in saline was measured for each blood sample from every donor between January 2008 and June 2012 in the Xi'an Blood Centre, China. D-negative results were confirmed by an indirect antiglobulin test and further investigated by adsorption-elution as required. The initial step of molecular analysis was RHD zygosity testing. Then RHD was detected by a sequence-specific polymerase chain reaction system for RHD(1227G>A), weak D type 15, and RHD(711delC) alleles for the samples carrying at least one RHD. For the remaining non-identified samples, ten RHD exons were amplified using a previously widely used RHD coding region

SUBMITTER: Ye SH 

PROVIDER: S-EPMC4111822 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature