Rab23 is a potential biological target for treating hepatocellular carcinoma.

ABSTRACT:

Aim

To elucidate the role of Rab23 in hepatocellular carcinoma (HCC) by assessing the expression of Rab23 in HCC tissue and in HCC cell lines.

Methods

Primary tumors (n = 100) were stained with Rab23 antibodies using immunohistochemistry and in situ hybridization in tissue microarrays. Relationships between gene expression and pathology parameters were analysed. The biological significance of Rab23 in Hep-3B cells was examined by knocking down Rab23 gene expression. We designed a pair of double-stranded RNAs against human rab23 and transfected siRNA into Hep-3B cells. Rab23 expression in these cells was examined using RT-PCR and Western blots. We investigated cell growth by MTT assays and fluorescence-activated cell sorting.

Results

High cytoplasmic and nuclear expres

SUBMITTER: Liu YJ 

PROVIDER: S-EPMC4146862 | biostudies-literature | 2007 Feb

REPOSITORIES: biostudies-literature