Proteomic profiling of serum from patients with tuberculosis.
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ABSTRACT: Background
Effective treatment and monitoring of tuberculosis (TB) requires biomarkers that can be easily evaluated in blood samples. The aim of this study was to analyze the serum proteome of patients with TB and to identify protein biomarkers for TB.Methods
Serum samples from 26 TB patients and 31 controls were analyzed by using nano-flow ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry in data-independent mode, and protein and peptide amounts were calculated by using a label-free quantitative approach. The generated data were analyzed by using principal component analysis and partial least squares discriminant analysis, a multivariate statistical method.Results
Of more than 500 proteins identified, alpha-1-antitrypsin was the most discriminative, which was 4.4 times higher in TB patients than in controls. Peptides from alpha-1-antitrypsin and antithrombin III increased in TB patients and showed a high variable importance in the projection scores and coefficient in partial least square discriminant analysis.Conclusions
Sera from patients with TB had higher alpha-1-antitrypsin levels than sera from control participants. Alpha-1-antitrypsin levels may aid in the diagnosis of TB.
SUBMITTER: Song SH
PROVIDER: S-EPMC4151002 | biostudies-literature | 2014 Sep
REPOSITORIES: biostudies-literature
Publications
Proteomic profiling of serum from patients with tuberculosis.
Song Sang Hoon SH Han Minje M Choi Yang Seon YS Dan Ki Soon KS Yang Man Gil MG Song Junghan J Park Sung Sup SS Lee Jae Ho JH
Annals of laboratory medicine 20140821 5
<h4>Background</h4>Effective treatment and monitoring of tuberculosis (TB) requires biomarkers that can be easily evaluated in blood samples. The aim of this study was to analyze the serum proteome of patients with TB and to identify protein biomarkers for TB.<h4>Methods</h4>Serum samples from 26 TB patients and 31 controls were analyzed by using nano-flow ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry in data-independent mode, and protein and pept ...[more]
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