Mechanisms of vitamin D₃ metabolite repression of IgE-dependent mast cell activation.

ABSTRACT:

Background

Mast cells have gained notoriety based on their detrimental contributions to IgE-mediated allergic disorders. Although mast cells express the vitamin D receptor (VDR), it is not clear to what extent 1α,25-dihydroxyvitamin D3 (1α,25[OH]2D3) or its predominant inactive precursor metabolite in the circulation, 25-hydroxyvitamin D3 (25OHD3), can influence IgE-mediated mast cell activation and passive cutaneous anaphylaxis (PCA) in vivo.

Objective

We sought to assess whether the vitamin D3 metabolites 25OHD3 and 1α,25(OH)2D3 can repress IgE-dependent mast cell activation through mast cell-25-hydroxyvitamin D-1α-hydroxylase (CYP27B1) and mast cell-VDR activity.

Methods

We measured the extent of vitamin D3 suppression of IgE-mediated mast cell degranulation and me

SUBMITTER: Yip KH 

PROVIDER: S-EPMC4154631 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature