The transcription factor Etv5 controls TH17 cell development and allergic airway inflammation.

ABSTRACT:

Background

The differentiation of TH17 cells, which promote pulmonary inflammation, requires the cooperation of a network of transcription factors.

Objectives

We sought to define the role of Etv5, an Ets-family transcription factor, in TH17 cell development and function.

Methods

TH17 development was examined in primary mouse T cells wherein Etv5 expression was altered by retroviral transduction, small interfering RNA targeting a specific gene, and mice with a conditional deletion of Etv5 in T cells. The direct function of Etv5 on the Il17 locus was tested with chromatin immunoprecipitation and reporter assays. The house dust mite-induced allergic inflammation model was used to test the requirement for Etv5-dependent TH17 functions in vivo.

Results

We identify E

SUBMITTER: Pham D 

PROVIDER: S-EPMC4209254 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature